Article

A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sites

2021-04-06

Abstract excerpt

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) systems have repeatedly shown to have excellent performance in nucleotide sensing applications 1–5 . High specificity and selectivity of Cas effector proteins is determined by the CRISPR RNA’s (crRNA’s) interchangeable spacer sequence, as well as position and number of mismatches between target sequence and the crRNA sequ...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b9be2941-09b5-5f18-929c-810bbf61cb04
DOI: 10.1101/2021.04.06.438612

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A CRISPR/Cas12a-assisted platform for identification and quantification of single CpG methylation sitesDOI 10.1101/2021.04.06.438612Read

Single nucleotide polymorphisms genotyping via an ultrasensitive CRISPR-based assaySemantic proximity Read

A Triple-Mismatch Differentiating assay exploiting activation and trans cleavage of CRISPR-Cas12a for mutation detection with ultra specificity and sensitivity.Semantic proximity Read

Detecting Melanocortin 1 Receptor Gene's SNPs by CRISPR/enAsCas12a.Semantic proximity Read

A detection method for the capture of genomic signatures: From disease diagnosis to genome editing.Semantic proximity Read

Signal logic gate mediated by a controllable CRISPR/Cas12a system for simultaneous detection of DNA mutation and methylation.Semantic proximity Read

Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing.Semantic proximity Read

Single-Nucleotide Variation Analysis in Oral Squamous Cell Carcinoma-Related ctDNA by dCas9/sgRNA Recognition-Mediated Proximity Ligation-Triggered Terminal Hairpin Formation and Self-Priming Amplification.Semantic proximity Read

A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration.Semantic proximity Read

Sequence-Specific Recognition of Double-Stranded Nucleic Acids by λ Exonuclease-Phosphorylated DNA Mediated Target InterrogationSemantic proximity Read

Detection of rare variant alleles using the AsCas12a double-stranded DNA trans-cleavage activity.Semantic proximity Read

Optimization and Clinical Application Potential of Single Nucleotide Polymorphism Detection Method Based on CRISPR/Cas12a and Recombinase Polymerase Amplification.Semantic proximity Read

Quantification of DNA methylation using methylation-sensitive restriction enzymes and multiplex digital PCRSemantic proximity Read