Article
A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration.
Nucleic acids research - 11 Nov 2022
Wu You, Luo Wang, Weng Zhi, Guo Yongcan, Yu Hongyan, Zhao Rong, Zhang Li, Zhao Jie, Bai Dan, Zhou Xi, Song Lin, Chen Kena, Li Junjie, Yang Yujun, Xie Guoming
Abstract excerpt
CRISPR (clustered regularly interspaced short palindromic repeats) technology has achieved great breakthroughs in terms of convenience and sensitivity; it is becoming the most promising molecular tool. However, only two CRISPR activation modes (single and double stranded) are available, and they have specificity and universality bottlenecks that limit the application of CRISPR technology in high-precision...
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