Article
Optimization and Clinical Application Potential of Single Nucleotide Polymorphism Detection Method Based on CRISPR/Cas12a and Recombinase Polymerase Amplification.
Analytical chemistry - 5 Nov 2024
Wang Xingyue, Yang Ting, Zhang Yunling, Zeng Zongyue, Wei Qiang, Chen Pu, Yang Shuangshuang, Huang Yunfei, Zhang Yongqi, Lu Hongling, Wu Linhong, Tang Dijiao, Yang Ping, Wang Xuechun, Liu Qing, Li Fan, Ling Chao, Huang Shifeng
Abstract excerpt
Conventional methods for detecting single nucleotide polymorphisms (SNPs) in clinical practice often require substantial time, labor, and specialized equipment, limiting their widespread application. To address this limitation, we refined our previous SNP detection method, IMAS-RPA [introducing an extra mismatched base adjacent to the single-base mutant site by recombinase polymerase amplification (RPA)],...
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