Article

Single nucleotide polymorphisms genotyping via an ultrasensitive CRISPR-based assay

2026-03-27

Abstract excerpt

<title>Abstract</title> <p>Single-nucleotide polymorphisms (SNPs) are crucial for dissecting genotype-phenotype associations, clinical diagnosis, and agricultural traits prediction. While CRISPR-based diagnostic tools enable rapid nucleic acid detection, their SNP discrimination ability is constrained by protospacer adjacent motif (PAM) requirements and inherent mismatch tolerance. Here, we found that PCR-generat...

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Literature Corpus work: 17224eab-cf76-5a46-a139-15a08e7cb20d
DOI: 10.21203/rs.3.rs-8857240/v1

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