Detection of rare variant alleles using the AsCas12a double-stranded DNA trans-cleavage activity.

Abstract excerpt

The sensitive and accurate detection of rare mutations has profound clinical implications; however, current methods require expensive instrumentation and are laborious and time-consuming. Thus, there is a need for a probe-based alternative that can effectively discriminate single-base mutations....

Topics

• Alleles
• Bacterial Proteins
• Biosensing Techniques
• CRISPR-Cas Systems
• DNA