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Article

Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing.

Molecular therapy : the journal of the American Society of Gene Therapy - 3 Mar 2021

Rabinowitz Roy, Offen Daniel

Abstract excerpt

The CRISPR-Cas system holds great promise in the treatment of diseases caused by genetic variations. The Cas protein, an RNA-guided programmable nuclease, generates a double-strand break at precise genomic loci. However, the use of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas system to distinguish between single-nucleotide variations is challenging. The promiscuity of the guide RNA...

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Identifiers

PMID: 33248248
DOI: 10.1016/j.ymthe.2020.11.009
PMCID: PMC7938333

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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