Single-Nucleotide Variation Analysis in Oral Squamous Cell Carcinoma-Related ctDNA by dCas9/sgRNA Recognition-Mediated Proximity Ligation-Triggered Terminal Hairpin Formation and Self-Priming Amplification.

Abstract excerpt

Circulating tumor DNA (ctDNA) represents a promising noninvasive biomarker for cancer diagnosis, including oral cancer. However, its clinical translation is currently limited by the lack of precise and reliable detection techniques. In this study, we developed a novel fluorescent biosensor for the detection of single-nucleotide variations in ctDNA, which integrates dual dCas9/sgRNA complexes for target...

Topics

• Humans
• Biosensing Techniques
• Circulating Tumor DNA
• Mouth Neoplasms
• Carcinoma, Squamous Cell
• Polymorphism, Single Nucleotide
• Nucleic Acid Amplification Techniques
• RNA, Guide, CRISPR-Cas Systems
• CRISPR-Cas Systems