Article

Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cells

2026-06-01

Abstract excerpt

Programmable intracellular production of short functional RNAs underlies diverse applications ranging from gene regulation to genome engineering, but is often constrained by cloning workflows, RNA synthesis, or viral-vector delivery. Here, we introduce a single-stranded DNA (ssDNA)-driven intracellular transcription strategy in which short ssDNA templates function as transcriptional substrates when paired with ort...

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Literature Corpus work: 4ae3ddf2-3040-5e37-be75-74ca996b577d
DOI: 10.64898/2026.05.30.729002

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