Article

A Robust and Scalable Workflow for the Production of Circular Single-Stranded DNA for Genome Engineering Applications

2026-08-15

Abstract excerpt

Circular single-stranded DNA (cssDNA) is a versatile biomolecule with applications spanning genome editing, DNA nanotechnology, synthetic biology, molecular diagnostics, and aptamer development. Compared with linear single-stranded DNA, cssDNA offers enhanced structural stability, resistance to in-cellulo degradation by exonucleases and enables the generation of long, sequence-defined DNA molecules that are diffic...

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Literature Corpus work: 1eeb1611-48ae-59b1-890d-a451d2ad09cd
DOI: 10.64898/2026.08.14.743880

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