Back to search

Article

Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosis

2020-03-24

Abstract excerpt

Recently, CRISPR-Cas technology has opened a new era of nucleic acid-based molecular diagnostics. However, current CRISPR-Cas-based nucleic acid biosensing has largely a lack of the quantitative detection ability and typically requires separate manual operations. Herein, we reported a dynamic aqueous multiphase reaction (DAMR) system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diag...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
5d7cf33e-ab32-5680-a0a5-c6851e44a48d
DOI
10.1101/2020.03.21.001644
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosisDOI 10.1101/2020.03.21.001644
Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Multifunctional CRISPR-Cas12aSemantic proximity
Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Dual-Functional CRISPR-Cas12aSemantic proximity
Microfluidics-Enabled Digital Isothermal Cas13a AssaySemantic proximity
Recent advances in isothermal amplification techniques coupled with clustered regularly interspaced short palindromic repeat/Cas systemsSemantic proximity
Development of a CRISPR/dCas9-based membrane-assisted colorimetric assay for detection of high-risk HPV16 and HPV18: a proof-of-concept study.Semantic proximity
Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensorsSemantic proximity
A Cas12a-based fluorescent microfluidic system for rapid on-site human papillomavirus diagnostics.Semantic proximity
Visual “Dam-Break Drainage” Nucleic Acid Chips: Family-Affordable Platform for Rapid and Ultrasensitive Pathogens DetectionSemantic proximity
Rapid, point-of-care molecular diagnostics with Cas13Semantic proximity
Ultrasensitive one-pot detection of monkeypox virus with RPA and CRISPR in a sucrose-aided multiphase aqueous systemSemantic proximity
Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA SensingSemantic proximity
Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18Semantic proximity
Select a neighboring publication to make it the new centre.