Article

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks

2017-07-09

Abstract excerpt

The RNA-guided DNA endonuclease Cas9 has emerged as a powerful new tool for genome engineering. Cas9 creates targeted double-strand breaks (DSBs) in the genome. Knock-in of specific mutations (precision genome editing) requires homology-directed repair (HDR) of the DSB by synthetic donor DNAs containing the desired edits, but HDR has been reported to be variably efficient. Here, we report that linear DNAs (single...

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Literature Corpus work: e69c3a86-978a-5134-aa0c-a1fbf375d94d
DOI: 10.1101/161109

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