Article

CRISPR-Cas9 mediated nuclear transport and genomic integration of nanostructured genes in human primary cells

2021-11-09

Abstract excerpt

DNA nanostructures are a promising tool for delivery of a variety of molecular payloads to cells. DNA origami structures, where 1000’s of bases are folded into a compact nanostructure, present an attractive approach to package genes; however, effective delivery of genetic material into cell nuclei has remained a critical challenge. Here we describe the use of DNA nanostructures encoding an intact human gene and a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6202a128-89b2-5aa5-aa2c-439c833e6ceb
DOI: 10.1101/2021.11.08.467750

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 mediated nuclear transport and genomic integration of nanostructured genes in human primary cellsDOI 10.1101/2021.11.08.467750Read

A Cas9 nanoparticle system with truncated Cas9 target sequences on DNA repair templates enhances genome targeting in diverse human immune cell typesSemantic proximity Read

DNA Origami Drives Gene Expression in a Human Cell Culture SystemSemantic proximity Read

Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPTSemantic proximity Read

Packaging and Uncoating of CRISPR/Cas Ribonucleoproteins for Efficient Gene Editing with Viral and Non-viral Extracellular NanoparticlesSemantic proximity Read

Piggybacking functionalized DNA nanostructures into live cell nucleiSemantic proximity Read

Circularization of genes and chromosome by CRISPR in human cellsSemantic proximity Read

Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9Semantic proximity Read

Delivery of genome editing tools: A promising strategy for HPV-related cervical malignancy therapySemantic proximity Read

Correlation of Precisely Fabricated Geometric Characteristics of DNA-Origami Nanostructures with Their Cellular Entry in Human Lens Epithelial CellsSemantic proximity Read

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome IntegrationSemantic proximity Read

Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and FunctionSemantic proximity Read

Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineeringSemantic proximity Read