Article

Protocol for rapid allelic discrimination qPCR genotyping of the <i>Winnie</i> mouse model

2026-02-18

Abstract excerpt

<h4>Summary</h4> Winnie mice are a widely used in vivo model of inflammatory bowel disease carrying a missense mutation in the Muc2 gene. Here, we present a protocol for genotyping Winnie mice using TaqMan allelic discrimination quantitative PCR. We describe tissue collection, rapid crude DNA extraction, probe-based amplification with dual-labeled fluorophores, and fluorescence-based genotype calling in a single...

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Literature Corpus work: b5debf18-6590-576e-b710-fe882b4fc6d4
DOI: 10.64898/2026.02.17.704640

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