Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model.

STAR protocols - 19 Jun 2026

Mansoori Behzad, Liang Chengyu

Abstract excerpt

Winnie mice are a widely used in vivo model of inflammatory bowel disease and carry a missense mutation in the Muc2 gene. Here, we present a protocol for genotyping Winnie mice using TaqMan allelic discrimination quantitative PCR. We describe tissue collection, rapid crude DNA extraction, probe-based amplification with dual-labeled fluorophores, and fluorescence-based genotype calling in a single reaction. This...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Disease Models, Animal
Share to Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genotyping Techniques

Share to Inflammatory Bowel Diseases

Share to Polymorphism, Single Nucleotide

Share to Real-Time Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 41966827
DOI: 10.1016/j.xpro.2026.104497
PMCID: PMC13092596

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model.PMID 41966827Read ↗

Protocol for rapid allelic discrimination qPCR genotyping of the <i>Winnie</i> mouse modelSemantic proximity Read ↗

Phenotyping of fecal microbiota of Winnie, a rodent model of spontaneous chronic colitis, reveals specific metabolic, genotoxic, and pro-inflammatory propertiesSemantic proximity Read ↗

A novel technique for rapid automated genotyping of DNA polymorphisms in the mouse.Semantic proximity Read ↗

Content and Performance of the MiniMUGA Genotyping Array: A New Tool To Improve Rigor and Reproducibility in Mouse Research.Semantic proximity Read ↗

A rapid, microplate SNP genotype assay for the leptinob allele.Semantic proximity Read ↗

Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation.Semantic proximity Read ↗

Content and performance of the MiniMUGA genotyping array, a new tool to improve rigor and reproducibility in mouse researchSemantic proximity Read ↗

Genotype identification of Math1/LacZ knockout mice based on real-time PCR with SYBR Green I dye.Semantic proximity Read ↗

Efficient detection, quantification and enrichment of subtle allelic alterations.Semantic proximity Read ↗

High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.Semantic proximity Read ↗

High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing.Semantic proximity Read ↗

Single-tube Ptprc SNP genotyping of JAXBoy (CD45.1) and C57BL/6J (CD45.2) mice by endpoint PCR and gel electrophoresis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.