High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.

doi:10.1101/gr.10.2.258

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Article

High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.

Genome research - 1 Feb 2000

Germer S, Holland M J, Higuchi R

Abstract excerpt

We have developed an accurate, yet inexpensive and high-throughput, method for determining the allele frequency of biallelic polymorphisms in pools of DNA samples. The assay combines kinetic (real-time quantitative) PCR with allele-specific amplification and requires no post-PCR processing. The relative amounts of each allele in a sample are quantified. This is performed by dividing equal aliquots of the pooled...

Topics

Animals
DNA
Gene Frequency
Humans
Mathematical Computing
Mice
Mice, Inbred A
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Inbred MRL lpr
Mice, Inbred NZB
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Templates, Genetic

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Identifiers

PMID: 10673283
DOI: 10.1101/gr.10.2.258
PMCID: PMC310828

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