Article
High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing.
Methods (San Diego, Calif.) - 1 Jul 2021
Gleeson Diane, Sethi Debarati, Platte Radka, Burvill Jonathan, Barrett Daniel, Akhtar Shaheen, Bruntraeger Michaela, Bottomley Joanna, Mouse Genetics Project Sanger, Bussell James, Ryder Edward
Abstract excerpt
Genotyping of knockout alleles in mice is commonly performed by end-point PCR or gene-specific/universal cassette qPCR. Both have advantages and limitations in terms of assay design and interpretation of results. As an alternative method for high-throughput genotyping, we investigated next generation sequencing (NGS) of PCR amplicons, with a focus on CRISPR-mediated exon deletions where antibiotic selection...
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