Article

Single-cell SNP-DNA sequencing precisely maps genotoxic events in CRISPR-edited primary cells

2025-04-11

Abstract excerpt

<title>Abstract</title> <p>Genome editing by CRISPR-Cas9 is promising for genetic disease and cancer gene therapy. However, safety concerns are still present, particularly the ON-target genotoxicity for protocols using nucleases. Quality control of edited cells before and after graft is mandatory, especially to assay megabase-scale genomic rearrangements induced at the targeted locus. These unintended events are...

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Literature Corpus work: b3a7babb-6e3e-5ded-8cb3-0f87f17e9a80
DOI: 10.21203/rs.3.rs-6170404/v1

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