Article

Long-read Individual-molecule Sequencing Reveals CRISPR-induced Genetic Heterogeneity in Human ESCs

2020-02-11

Abstract excerpt

Accurately quantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4×10 −5 , using short- or long-read sequencing. It enables base-resolution haplotype-resolved quantitative characterization of rare variants. It provi...

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Literature Corpus work: b0bc2503-e440-5455-986f-6469f808f964
DOI: 10.1101/2020.02.10.942151

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