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Article

Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment.

Nature communications - 10 Jan 2026

Boutin Julian, Fayet Sabrina, Marin Victor, Bergès Camille, Riandière Maude, Toutain Jérôme, Lamrissi-Garcia Isabelle, Thibault Chloé, Cappellen David, Dabernat Sandrine, Poulet Arthur, Francillette Maëla, Droin Nathalie, Debeissat Christelle, Brunet de la Grange Philippe, Moreau-Gaudry François, Bedel Aurélie

Abstract excerpt

Genome editing by CRISPR-Cas9-nuclease is promising for gene therapy. However, safety concerns remain. Monitoring ON-target genotoxicity is essential, especially to assay megabasic rearrangements at the targeted locus. Here, we developed a combined single-cell resolution approach with DNA sequencing focused on single nucleotide polymorphism (scSNP-DNAseq), micronuclei and LOH cytometry-reporter assays. This...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41519897
DOI: 10.1038/s41467-025-68177-3
PMCID: PMC12881610

Genes

HBG1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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