Article

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cells

2026-06-15

Abstract excerpt

<h4>Abstract/Resume</h4> DNA-PK inhibitors (DNA-PKi) have emerged as potent enhancers of homology-directed repair (HDR) in genome editing, however, recent studies have reported that such inhibitors can induce large-scale structural variations (SVs) raising safety concerns. To investigate factors influencing SV induction in human hematopoietic stem and progenitor cells (HSPCs), we employed a combination of on-targ...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8339db29-afd1-5358-9355-aeb381f3c95e
DOI: 10.64898/2026.06.13.732078

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cellsDOI 10.64898/2026.06.13.732078Read

GREPore-seq: A Robust Workflow to Detect Changes after Gene Editing through Long-range PCR and Nanopore SequencingSemantic proximity Read

Single-cell SNP-DNA sequencing precisely maps genotoxic events in CRISPR-edited primary cellsSemantic proximity Read

DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs.Semantic proximity Read

CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cellsSemantic proximity Read

MEGA dPCR reveals chromosomal aberrations, NHEJ precise repair, recurrent nuclease cleavage and DSB half-lifeSemantic proximity Read

A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage.Semantic proximity Read

Detection of deleterious on-target effects after HDR-mediated CRISPR editingSemantic proximity Read

Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment.Semantic proximity Read

Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterationsSemantic proximity Read

Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells.Semantic proximity Read

Enabling large-scale genome editing by reducing DNA nickingSemantic proximity Read

Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineeringSemantic proximity Read