Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs.

Genome biology - 24 Aug 2020

Bi Chongwei, Wang Lin, Yuan Baolei, Zhou Xuan, Li Yu, Wang Sheng, Pang Yuhong, Gao Xin, Huang Yanyi, Li Mo

Abstract excerpt

Quantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for single-base-resolution haplotype-resolved quantitative characterization of diverse types of rare variants, with frequency as low as 4 × 10-5, using both short- or long-read sequencing platforms. It provides the first quantitative...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to Gene Editing

Share to Gene Knock-In Techniques

Share to Genetic Heterogeneity

Share to Human Embryonic Stem Cells

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 32831134
DOI: 10.1186/s13059-020-02143-8
PMCID: PMC7444080

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs.PMID 32831134Read ↗

Long-read Individual-molecule Sequencing Reveals CRISPR-induced Genetic Heterogeneity in Human ESCsSemantic proximity Read ↗

Precise measurement of CRISPR genome editing outcomes through single-cell DNA sequencingSemantic proximity Read ↗

Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells.Semantic proximity Read ↗

Detection of deleterious on-target effects after HDR-mediated CRISPR editingSemantic proximity Read ↗

Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping.Semantic proximity Read ↗

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingSemantic proximity Read ↗

Global-scale CRISPR gene editor specificity profiling by ONE-seq identifies population-specific, variant off-target effectsSemantic proximity Read ↗

Analyzing long-read CRISPR experiments with CRISPRLungoSemantic proximity Read ↗

Single-cell SNP-DNA sequencing precisely maps genotoxic events in CRISPR-edited primary cellsSemantic proximity Read ↗

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cellsSemantic proximity Read ↗

Genome-Wide Analysis of Off-Target CRISPR/Cas9 Activity in Single-Cell-Derived Human Hematopoietic Stem and Progenitor Cell ClonesSemantic proximity Read ↗

scSNV-seq: high-throughput phenotyping of single nucleotide variants by coupled single-cell genotyping and transcriptomics.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.