Article

Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis

2023-12-22

Abstract excerpt

<title>Abstract</title> <p><italic>Trans</italic>-cleavage rates of Cas endonucleases such as Cas12a remain limited despite optimisation efforts. We found that Cas12a ribonucleoproteins poorly bind to, and are only minimally activated by small circular DNA nanostructures which partially match the gRNA sequence. However, activation is restored when the nanostructure is linearised. Building on this finding, we esta...

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Literature Corpus work: af889275-9817-54a2-82d8-bb6d130b2aaf
DOI: 10.21203/rs.3.rs-2586294/v1

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