Article
Enhanced trans-cleavage activity using CRISPR-Cas12a variant designed to reduce steric inhibition by cis-cleavage products.
Biosensors & bioelectronics - 1 Jan 2025
Hwang Injoo, Song Yo Han, Lee Sanghwa
Abstract excerpt
The CRISPR-Cas12a system has emerged as a promising tool for molecular diagnostics due to its indiscriminate trans-ssDNase activity. However, the sensitivity of Cas12a-based diagnostics remains insufficient for clinical use without a pre-amplification step such as loop-mediated isothermal amplifi...
Topics
- CRISPR-Cas Systems
- Biosensing Techniques
- Hepatitis B virus
- Humans
- CRISPR-Associated Proteins
- Endodeoxyribonucleases
- DNA, Viral
- Bacterial Proteins
- Mutation
- Hepatitis B