Article

Kinetic Modeling of Target-Amplification-Free CRISPR-Cas-Based Autocatalysis Reactions

2026-03-04

Abstract excerpt

CRISPR-Cas-based diagnostics utilize the Cas enzyme’s trans-cleavage activity to generate signal and have become popular platforms for sensitive nucleic acid detection. Recently, autocatalytic systems have been demonstrated to improve the time to response and sensitivity in some cases. However, mechanistic description of these assays is limited and optimization relies on simple trial-and-error. In this work, we pr...

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Literature Corpus work: 0a523967-1120-5781-afc5-27c2f85a9044
DOI: 10.64898/2026.03.03.709462

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