Back to search

Article

Hairpin-locker mediated CRISPR/Cas tandem system for ultrasensitive detection of DNA without pre-amplification

2024-03-05

Abstract excerpt

Achieving ultra-sensitive detection of DNA is of paramount importance in the field of molecular analytics. Conventional amplification technologies such as polymerase chain reaction (PCR) currently play a leading role in ultrasensitive DNA detection. However, amplicon contamination common in these techniques may lead to false positives. To date, CRISPR-associated nucleases (type V & VI) with their programmable clea...

Identifiers and source

Literature Corpus work
7df2b14c-bbb8-5260-90f0-59bf6ca30252
DOI
10.1101/2024.03.05.583466
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Hairpin-locker mediated CRISPR/Cas tandem system for ultrasensitive detection of DNA without pre-amplificationDOI 10.1101/2024.03.05.583466
Programmable Biosensors Based on RNA-Guided CRISPR/Cas EndonucleaseSemantic proximity
CRISPR/Cas-based nucleic acid detection strategies: Trends and challengesSemantic proximity
Nucleic Acid Detection Using CRISPR/Cas Biosensing TechnologiesSemantic proximity
Amplification-free RNA detection with CRISPR–Cas13Semantic proximity
From RNA to DNA: CRISPR/LbuCas13a Demonstrates Exceptional Single-Nucleotide SpecificitySemantic proximity
A Universal Proximity CRISPR Cas12a Assay for Ultrasensitive Detection of Nucleic Acids and ProteinsSemantic proximity
CRISPR-Cas13a system: A novel tool for molecular diagnosticsSemantic proximity
CRISPR-Cas target recognition for sensing viral and cancer biomarkersSemantic proximity
A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration.Semantic proximity
STAR-CRISPR: a one-pot ultraspecific CRISPR strategy for rapid, visualized SNV detection and genotyping in point-of-care diagnostics.Semantic proximity
CRISPR-cas technology: A key approach for SARS-CoV-2 detectionSemantic proximity
The CRISPR–Cas toolbox for analytical and diagnostic assay developmentSemantic proximity
Select a neighboring publication to make it the new centre.