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Article

Repurpose Type I-A CRISPR-Cas3 for a Robust HPV Diagnosis

2023-10-20

Abstract excerpt

<title>Abstract</title> <p>R-loop-triggered collateral single-stranded DNA (ssDNA) nuclease activity within Class 1 Type I CRISPR-Cas systems holds immense potential for nucleic acid detection. However, the hyperactive ssDNase activity of Cas3 introduces unwanted noise and false-positive results. In this study, we identified a novel Type I-A Cas3 variant derived from Thermococcus siculi, which remains in an auto-...

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Identifiers and source

Literature Corpus work
e1edbca4-d108-5416-98bc-0a4d61f0d54d
DOI
10.21203/rs.3.rs-3354708/v1
Open publication

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Repurpose Type I-A CRISPR-Cas3 for a Robust HPV DiagnosisDOI 10.21203/rs.3.rs-3354708/v1
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