Article

DNA damage hypersensitivity in chicken primordial germ cells limits CRISPR-Cas9 editing and supports an shRNA-based screening approach

2025-09-20

Abstract excerpt

Chickens ( Gallus gallus ) are uniquely suited for germline studies because their primordial germ cells (PGCs) can be propagated long-term in vitro and used for germline transmission. To develop a loss-of-function screening platform in chicken PGCs, we compared three perturbation methods: CRISPR/Cas9 knockout, CRISPR interference (CRISPRi), and shRNA-mediated knockdown. We found that CRISPR/Cas9 editing causes se...

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Literature Corpus work: ad9dc689-9ed3-511c-994b-ce166d4d048c
DOI: 10.1101/2025.09.17.676926

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