CRISPR-Cas9 Mutagenesis in Xenopus tropicalis for Phenotypic Analyses in the F0 Generation and Beyond.

Abstract excerpt

CRISPR-Cas9 mutagenesis is being widely used to create targeted loss-of-function mutations in the diploid frog Xenopus tropicalis Here we describe a simple mutagenesis protocol using microinjection of Cas9 protein or mRNA, together with synthetic guide RNAs (sgRNAs) targeting specific DNA sequences, into the early embryo. Cas9-catalyzed double-strand breaks undergo error-prone repair, resulting in production of...

Topics

• Animals
• CRISPR-Cas Systems
• Chromosomes, Human, Y
• Gene Editing
• Humans
• Male
• Mosaicism
• Mutagenesis
• Phenotype
• Xenopus