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Article

Efficient TALEN-mediated gene targeting of chicken primordial germ cells.

Development (Cambridge, England) - 1 Mar 2017

Taylor Lorna, Carlson Daniel F, Nandi Sunil, Sherman Adrian, Fahrenkrug Scott C, McGrew Michael J

Abstract excerpt

In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation. We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies. Large genetic deletions of 30 kb...

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Identifiers

PMID: 28174243
DOI: 10.1242/dev.145367
PMCID: PMC5374353

Genes

DDX4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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