Article

eSPLIT and iSWAP: CRISPR-Mediated Conditional Allele Engineering with Short Artificial Introns

2025-12-24

Abstract excerpt

<h4>ABSTRACT</h4> Engineering conditional alleles remains a major challenge in functional genomics. Short Artificial Introns (SAIs) have emerged as powerful tools to simplify allele design and characterization, yet practical guidelines for their implementation remain limited. Here, we describe two streamlined strategies, eSPLIT (exon split) and iSWAP (intron swap), that enable efficient generation of conditional...

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Literature Corpus work: ab104a08-b671-5614-8286-19d7117d503f
DOI: 10.64898/2025.12.22.696077

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