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Article

A scalable two-step genome editing strategy for generating full-length gene-humanized mice at diverse genomic loci.

Nature communications - 14 Jan 2026

Taguchi Jumpei, Kikuchi Mio, Jeon Hyojung, Shimizu Ryo, Mori Hideto, Ikawa Masahito, Yamada Yasuhiro, Sato Kei, Ikeda Terumasa, Yamazaki Satoshi, Ozawa Manabu

Abstract excerpt

Full-length gene-humanized mice engineered by completely replacing mouse loci with human counterparts, including untranslated and regulatory regions, provide a robust in vivo platform for human gene function studies. However, reliably humanizing large genomic regions remains challenging due to limited DNA insert sizes, complex protocols, and specialized material requirements. This study introduces a streamlined...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41535270
DOI: 10.1038/s41467-025-67900-4
PMCID: PMC12804900

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