Article
Efficient nonmeiotic allele introgression in livestock using custom endonucleases.
Proceedings of the National Academy of Sciences of the United States of America - 8 Oct 2013
Tan Wenfang, Carlson Daniel F, Lancto Cheryl A, Garbe John R, Webster Dennis A, Hackett Perry B, Fahrenkrug Scott C
Abstract excerpt
We have expanded the livestock gene editing toolbox to include transcription activator-like (TAL) effector nuclease (TALEN)- and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-stimulated homology-directed repair (HDR) using plasmid, rAAV, and oligonucleotide templates. Toward the genetic dehorning of dairy cattle, we introgressed a bovine POLLED allele into horned bull fibroblasts. Single...
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