Article

Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells

2022-03-12

Abstract excerpt

Functional annotation of the vast noncoding landscape of the diploid human genome still remains a major challenge of genomic research. An efficient, scarless, biallelic, and gene-wide mutagenesis approach is needed for direct investigation of the functional significance of endogenous long introns in gene regulation. Here we established a genome substitution platform, the Universal Knock-in System or UKiS, that mee...

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Identifiers and source

Literature Corpus work: 76a87562-1a9b-5103-9a5f-846b165ef6ec
DOI: 10.1101/2022.03.09.482138

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