Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.

BMC biology - 21 Jun 2018

Lanza Denise G, Gaspero Angelina, Lorenzo Isabel, Liao Lan, Zheng Ping, Wang Ying, Deng Yu, Cheng Chonghui, Zhang Chuansheng, Seavitt John R, DeMayo Francesco J, Xu Jianming, Dickinson Mary E, Beaudet Arthur L, Heaney Jason D

Abstract excerpt

BACKGROUND: The International Mouse Phenotyping Consortium is generating null allele mice for every protein-coding gene in the genome and characterizing these mice to identify gene-phenotype associations. While CRISPR/Cas9-mediated null allele production in mice is highly efficient, generation of conditional alleles has proven to be more difficult. To test the feasibility of using CRISPR/Cas9 gene editing to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mice, Knockout
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Single-Stranded

Share to Gene Editing

Share to Loss of Function Mutation

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 29925370
DOI: 10.1186/s12915-018-0529-0
PMCID: PMC6011517

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.PMID 29925370Read ↗

Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants.Semantic proximity Read ↗

CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells.Semantic proximity Read ↗

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome.Semantic proximity Read ↗

Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes.Semantic proximity Read ↗

<i>Easi</i> -CRISPR: Efficient germline modification with long ssDNA donorsSemantic proximity Read ↗

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR/Cas9-mediated genome editing eventsSemantic proximity Read ↗

A resource of targeted mutant mouse lines for 5,061 genesSemantic proximity Read ↗

A conditional knockout resource for the genome-wide study of mouse gene function.Semantic proximity Read ↗

Recovering a Targeted Mutation in Mice from Embryonic Stem Cell Chimeras or CRISPR-Cas Founders.Semantic proximity Read ↗

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein.Semantic proximity Read ↗

Generation of multipurpose alleles for the functional analysis of the mouse genome.Semantic proximity Read ↗

Genetic engineering as a tool for the generation of mouse models to understand disease phenotypes and gene function.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.