Article
Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci.
BMC biotechnology - 12 May 2022
Bernas Guillaume, Ouellet Mariette, Barrios Andréa, Jamann Hélène, Larochelle Catherine, Lévy Émile, Schmouth Jean-François
Abstract excerpt
BACKGROUND: The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i.e. two loxP sites flanking an exon or a critical DNA sequence of interest) are amongst the most widely requested project type that are challenging to generate as they require simultaneous...
Topics
Join the communities discussing this publication.