Article
Conditionals by inversion provide a universal method for the generation of conditional alleles.
Proceedings of the National Academy of Sciences of the United States of America - 20 Aug 2013
Economides Aris N, Frendewey David, Yang Peter, Dominguez Melissa G, Dore Anthony T, Lobov Ivan B, Persaud Trikaldarshi, Rojas Jose, McClain Joyce, Lengyel Peter, Droguett Gustavo, Chernomorsky Rostislav, Stevens Sean, Auerbach Wojtek, Dechiara Thomas M, Pouyemirou William, Cruz Joseph M, Feeley Kieran, Mellis Ian A, Yasenchack Jason, Hatsell Sarah J, Xie Liqin, Latres Esther, Huang Lily, Zhang Yuhong, Pefanis Evangelos, Skokos Dimitris, Deckelbaum Ron A, Croll Susan D, Davis Samuel, Valenzuela David M, Gale Nicholas W, Murphy Andrew J, Yancopoulos George D
Abstract excerpt
Conditional mutagenesis is becoming a method of choice for studying gene function, but constructing conditional alleles is often laborious, limited by target gene structure, and at times, prone to incomplete conditional ablation. To address these issues, we developed a technology termed conditionals by inversion (COIN). Before activation, COINs contain an inverted module (COIN module) that lies inertly within the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.