Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice.

STAR protocols - 17 Mar 2023

Cassidy Annelise, Pelletier Stephane

Abstract excerpt

In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to CRISPR-Cas Systems
Share to Exons

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Editing

Share to Introns

Source

Open in PubMed Open full text

Identifiers

PMID: 36853660
DOI: 10.1016/j.xpro.2023.102116
PMCID: PMC9943854

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 11 closest

CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice.PMID 36853660Read ↗

eSPLIT and iSWAP: CRISPR-Mediated Conditional Allele Engineering with Short Artificial IntronsSemantic proximity Read ↗

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.Semantic proximity Read ↗

Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes.Semantic proximity Read ↗

Generation of Casp8 FL122/123GG Mice Using CRISPR-Cas9 Technology.Semantic proximity Read ↗

Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR.Semantic proximity Read ↗

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles.Semantic proximity Read ↗

One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster.Semantic proximity Read ↗

Rescuing lethal phenotypes induced by disruption of genes in mice: a review of novel strategies.Semantic proximity Read ↗

GeneFix-AI: AI-Powered CRISPR-Cas9 System for Real-Time Detection and Correction of Mutations in Non-Human SpeciesSemantic proximity Read ↗

CLICK: one-step generation of conditional knockout mice.Semantic proximity Read ↗

A genotype-phenotype screening system using conditionally immortalized immature dendritic cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.