Article

Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in <i>Ciona</i>

2016-02-28

Abstract excerpt

The CRISPR/Cas9 system has emerged as an important tool for various genome engineering applications. A current obstacle to high throughput applications of CRISPR/Cas9 is the imprecise prediction of highly active single guide. RNAs (sgRNAs). We previously implemented the CRISPR/Cas9 system to induce tissue-specific mutations in the tunicate Ciona . In the present study, we designed and tested 83 single guide RNA (...

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Literature Corpus work: a8f9c02b-dec2-5c59-862e-3a5255857eac
DOI: 10.1101/041632

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