Article

Genome and epigenome engineering CRISPR toolkit for probing <i>in vivo cis</i> -regulatory interactions in the chicken embryo

2017-05-08

Abstract excerpt

CRISPR-Cas9 genome engineering has revolutionised all aspects of biological research, with epigenome engineering transforming gene regulation studies. Here, we present a highly efficient toolkit enabling genome and epigenome engineering in the chicken embryo, and demonstrate its utility by probing gene regulatory interactions mediated by neural crest enhancers. First, we optimise efficient guide-RNA expression fro...

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Literature Corpus work: b219c626-1aae-50a1-8dc8-8fef5fcaa7f2
DOI: 10.1101/135525

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