Article
In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system.
Scientific reports - 30 Aug 2016
Narayanan Anand, Hill-Teran Guillermina, Moro Albertomaria, Ristori Emma, Kasper Dionna M, A Roden Christine, Lu Jun, Nicoli Stefania
Abstract excerpt
A large number of microRNAs (miRNAs) are grouped into families derived from the same phylogenetic ancestors. miRNAs within a family often share the same physiological functions despite differences in their primary sequences, secondary structures, or chromosomal locations. Consequently, the generation of animal models to analyze the activity of miRNA families is extremely challenging. Using zebrafish as a model...
Topics
- Animals
- CRISPR-Cas Systems
- Chromosomes
- Genome
- MicroRNAs
- Multigene Family
- Mutagenesis
- Mutation
- Zebrafish