Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona.

Abstract excerpt

The CRISPR/Cas9 system has emerged as an important tool for various genome engineering applications. A current obstacle to high throughput applications of CRISPR/Cas9 is the imprecise prediction of highly active single guide RNAs (sgRNAs). We previously implemented the CRISPR/Cas9 system to induce tissue-specific mutations in the tunicate Ciona. In the present study, we designed and tested 83 single guide RNA...

Topics

• Animals
• Base Sequence
• CRISPR-Cas Systems
• Ciona intestinalis
• Embryo, Nonmammalian
• Gene Editing
• Gene Expression Regulation, Developmental
• Gene Targeting
• High-Throughput Nucleotide Sequencing
• Mutagenesis