Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos.

doi:10.1038/cr.2013.45

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos.

Cell research - 1 Apr 2013

Chang Nannan, Sun Changhong, Gao Lu, Zhu Dan, Xu Xiufei, Zhu Xiaojun, Xiong Jing-Wei, Xi Jianzhong Jeff

Abstract excerpt

Recent advances with the type II clustered regularly interspaced short palindromic repeats (CRISPR) system promise an improved approach to genome editing. However, the applicability and efficiency of this system in model organisms, such as zebrafish, are little studied. Here, we report that RNA-guided Cas9 nuclease efficiently facilitates genome editing in both mammalian cells and zebrafish embryos in a simple...

Topics

Animals
Base Sequence
Embryo, Nonmammalian
Fish Proteins
GATA Transcription Factors
GATA5 Transcription Factor
Genome
Molecular Sequence Data
Mutation
RNA Editing
Reverse Genetics
Ribonucleases
Zebrafish
Zebrafish Proteins
RNA, Small Untranslated

Source

Open in PubMed Open full text

Identifiers

PMID: 23528705
DOI: 10.1038/cr.2013.45
PMCID: PMC3616424

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics