Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool.

PloS one - 1 Jan 2017

Donovan Katherine F, Hegde Mudra, Sullender Meagan, Vaimberg Emma W, Johannessen Cory M, Root David E, Doench John G

Abstract excerpt

CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. Based on unexpected results from a genome-wide screen, we developed a CRISPR/Cas9-mediated approach to mutagenesis, exploiting the allelic diversity generated by error-prone non-homologous end-joining (NHEJ) to identify novel gain-of-function and drug resistant alleles of the MAPK signaling pathway genes MEK1 and BRAF. We define the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Clustered Regularly Interspaced Short Palindromic Repeats
Share to DNA End-Joining Repair

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to MAP Kinase Kinase 1

Share to Protein Engineering

Share to RNA, Guide, CRISPR-Cas Systems

Share to Sulfonamides

Share to Transduction, Genetic

Share to Vemurafenib

Source

Open in PubMed Open full text

Identifiers

PMID: 28118392
DOI: 10.1371/journal.pone.0170445
PMCID: PMC5261743

Genes

BRAF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool.PMID 28118392Read ↗

The application of CRISPR/Cas9–based genome-wide screening to disease researchSemantic proximity Read ↗

Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines.Semantic proximity Read ↗

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens.Semantic proximity Read ↗

Recent advances in CRISPR-based functional genomics for the study of disease-associated genetic variants.Semantic proximity Read ↗

Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomesSemantic proximity Read ↗

Engineering CRISPR/Cas9 therapeutics for cancer precision medicineSemantic proximity Read ↗

CRISPR-Cas9 Causes Chromosomal Instability and Rearrangements in Cancer Cell Lines, Detectable by Cytogenetic Methods.Semantic proximity Read ↗

Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes.Semantic proximity Read ↗

Prime Editing Driven Functional Genomics: Bridging Genotype to Phenotype in the Post-Genomic Era.Semantic proximity Read ↗

CRISPR-based knock-in mutagenesis of the pioneer transcription factor FOXA1: optimization of strategies for multi-allelic proteins in cancer cells.Semantic proximity Read ↗

Highly parallel genome variant engineering with CRISPR-Cas9.Semantic proximity Read ↗

GenEditID: an open-access platform for the high-throughput identification of CRISPR edited cell clonesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.