Article

Directed evolution of an m6A eraser for site-selective epitranscriptome editing

2025-11-04

Abstract excerpt

<title>Abstract</title> <p>N6-methyladenosine (m6A) is a critical RNA modification, but tools to manipulate it in cells remain limited. Current m6A demethylases (FTO and ALKBH5) exhibit low catalytic efficiency, hindering their applications in epitranscriptomic research and biotechnology. Here, we develop a fluorescence-based directed evolution platform that enables rapid selection of m6A demethylases with enhanc...

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Literature Corpus work: a66ad62f-7180-5902-8f36-e312386b8590
DOI: 10.21203/rs.3.rs-7958216/v1

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