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Advanced eTAM-seq enables high-fidelity, low-input N 6 -methyladenosine profiling in human cells and embryonic mouse tissues

2025-05-15

Abstract excerpt

Functional dissection of N6-methyladenosine (m 6 A), the most abundant internal messenger RNA modification in mammals, demands quantitative, scalable detection technology. We previously reported eTAM-seq, which supports transcriptome-wide quantification of m 6 A by enzyme-assisted adenosine deamination. While effective, TadA8.20—the enzyme used in our first-generation technology—is sensitive not only to m 6 A b...

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Literature Corpus work: 6c6cfdcb-03da-5480-915d-03335dc7650d
DOI: 10.1101/2025.05.11.653357

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