Article

Discovery of novel DNA cytosine deaminase activities enables a nondestructive single-enzyme methylation sequencing method for base resolution high-coverage methylome mapping of cell-free and ultra-low input DNA

2023-06-30

Abstract excerpt

Cytosine deaminases have important uses in the detection of epigenetic modifications and in genome editing. However, the range of applications of deaminases is limited by a small number of well characterized enzymes. To expand the toolkit of deaminases, we developed an in-vitro approach that bypasses a major hurdle with their severe toxicity in expression hosts. We systematically assayed the activity of 175 putati...

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Literature Corpus work: aebabb78-b0ef-5ab7-83f6-f61d7a931dbb
DOI: 10.1101/2023.06.29.547047

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