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Article

A high-throughput screening method for evolving a demethylase enzyme with improved and new functionalities.

Nucleic acids research - 18 Mar 2021

Wang Yuru, Katanski Christopher D, Watkins Christopher, Pan Jessica N, Dai Qing, Jiang Zhuoxun, Pan Tao

Abstract excerpt

AlkB is a DNA/RNA repair enzyme that removes base alkylations such as N1-methyladenosine (m1A) or N3-methylcytosine (m3C) from DNA and RNA. The AlkB enzyme has been used as a critical tool to facilitate tRNA sequencing and identification of mRNA modifications. As a tool, AlkB mutants with better reactivity and new functionalities are highly desired; however, previous identification of such AlkB mutants was based...

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PMID: 33337498
DOI: 10.1093/nar/gkaa1213
PMCID: PMC7968990

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