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Article

CRISPR-mediated genetic interaction profiling identifies RNA binding proteins controlling metazoan fitness.

eLife - 18 Jul 2017

Norris Adam D, Gracida Xicotencatl, Calarco John A

Abstract excerpt

Genetic interaction screens have aided our understanding of complex genetic traits, diseases, and biological pathways. However, approaches for synthetic genetic analysis with null-alleles in metazoans have not been feasible. Here, we present a CRISPR/Cas9-based Synthetic Genetic Interaction (CRISPR-SGI) approach enabling systematic double-mutant generation. Applying this technique in Caenorhabditis elegans, we...

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Identifiers

PMID: 28718764
DOI: 10.7554/eLife.28129
PMCID: PMC5544425

Genes

MBNL1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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