Article
CRISPR-mediated genetic interaction profiling identifies RNA binding proteins controlling metazoan fitness.
eLife - 18 Jul 2017
Norris Adam D, Gracida Xicotencatl, Calarco John A
Abstract excerpt
Genetic interaction screens have aided our understanding of complex genetic traits, diseases, and biological pathways. However, approaches for synthetic genetic analysis with null-alleles in metazoans have not been feasible. Here, we present a CRISPR/Cas9-based Synthetic Genetic Interaction (CRISPR-SGI) approach enabling systematic double-mutant generation. Applying this technique in Caenorhabditis elegans, we...
Topics
- Animals
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Clustered Regularly Interspaced Short Palindromic Repeats
- Epistasis, Genetic
- High-Throughput Screening Assays
- Mutation
- Phenotype
- RNA Interference
- RNA-Binding Proteins