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High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

Cell - 3 Dec 2015

Hart Traver, Chandrashekhar Megha, Aregger Michael, Steinhart Zachary, Brown Kevin R, MacLeod Graham, Mis Monika, Zimmermann Michal, Fradet-Turcotte Amelie, Sun Song, Mero Patricia, Dirks Peter, Sidhu Sachdev, Roth Frederick P, Rissland Olivia S, Durocher Daniel, Angers Stephane, Moffat Jason

Abstract excerpt

The ability to perturb genes in human cells is crucial for elucidating gene function and holds great potential for finding therapeutic targets for diseases such as cancer. To extend the catalog of human core and context-dependent fitness genes, we have developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Using an improved...

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PMID: 26627737
DOI: 10.1016/j.cell.2015.11.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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