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Minimized combinatorial CRISPR screens identify genetic interactions in autophagy

16 Apr 2021

Abstract excerpt

Combinatorial CRISPR-Cas screens have advanced the mapping of genetic interactions, but their experimental scale limits the number of targetable gene combinations. Here, we describe 3Cs multiplexing, a rapid and scalable method to generate highly diverse and uniformly distributed combinatorial CRISPR libraries. We demonstrate that the library distribution skew is the critical determinant of its required screening...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 33956155
DOI: 10.1093/nar/gkab309

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